Part:BBa_K2514001:Design
pSB1C3-miR-21-sponge-2s
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Pervious studies indicated sponges containing bulged sites that are mispaired opposite miRNA are more effective than perfectly complementary sponges for individual miRNAs. And miRNA sponges contained different binding sites have different efficiency. we designed two different mir-21 sponges with different binding sits to test the efficiency of sponges. One mir-21 sponge contains two miR-21 binding sites with 3-nt spacers for bulged sites. Another one mir-21 sponge contains six miR-21 binding sites with 3-nt spacers for bulged sites. We wanted to compare the accuracy and the effectiveness of different mir-21 sponges contained different binding sites for measuring the expression of miR-21 in colorectal cancer cells.
Source
We designed mir-21 sponges contains two miR-21 binding sites with 3-nt spacers for bulged sites based on the sequence of hsa-miR-21 according to the previous study (Ebert MS, Neilson JR, Sharp PA. MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells. Nat Methods. 2007 Sep;4(9):721-6)